Single particle electron microscopy of native cell extracts
Rapid developments in cryo-EM continue to open new possibilities for structural studies of biological macromolecules. One key advantage of cryo-EM is that particles do not need to be homogeneous. Taken to an extreme, can single particle cryo-EM be used for solving structures from mixtures as complex as cell lysate? Towards this goal, I will share our approach for computationally sorting particles from a mixture prior to 3D classification using red blood cells as a model system.
Meeting ID: 990 3706 2172